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- Title
Vertical Transmission of (Microspora) within the Eggs of the Golden Shiner.
- Authors
Phelps, Nicholas B. D.; Goodwin, Andrew E.
- Abstract
Fertilized eggs collected from broodfish infected by were tested by quantitative polymerase chain reaction (PCR) and found to be positive for the genome at 7.77 × 102 to 3.26 × 107 copies per microgram of host DNA. Fry hatched from these eggs contained from 1.37 × 102 to 9.89 × 106 copies of the genome per microgram of host DNA. Surface treatments of fertilized eggs with 150 mg formalin/L (used by farms as a fungicide) or a 1.5% solution of sodium sulfite (which removes the adhesive egg matrix) did not reduce vertical transmission to fry. Treatment of eggs with a 10% solution of bleach or a proprietary commercial DNA denaturant did not reduce the number of egg-associated copies of the genome. Histology of ovaries of infected fish demonstrated spores within the oocytes. However, no spores were observed by histology in positive fry hatched from infected eggs. The PCR and histological demonstration of the presence of spores in oocytes and fry, and the failure of strong DNA denaturants to reduce egg-associated copies, give evidence that is vertically transmitted within eggs.
- Publication
Journal of Aquatic Animal Health, 2008, Vol 20, Issue 1, p45
- ISSN
0899-7659
- Publication type
Article
- DOI
10.1577/H07-029.1