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- Title
Interlaboratory comparison of real-time polymerase chain reaction methods to detect Coxiella burnetii, the causative agent of Q fever.
- Authors
Jones, Rebecca M.; Hertwig, Stefan; Pitman, James; Vipond, Richard; Aspán, Anna; Bölske, Göran; McCaughey, Conall; McKenna, James P.; van Rotterdam, Bart J.; de Bruin, Arnout; Ruuls, Robin; Buijs, Rob; Roest, Hendrik-Jan; Sawyer, Jason
- Abstract
The article discusses a ring-trial involving 7 European testing centers in Great Britain, Sweden and Germany to compare several real-time polymerase chain reaction (PCR) methods in the detection of Coxiella burnetii. Ten nucleic acid samples were received by each testing center. It indicates that real-time PCR assays that target multiple copy genes are more sensitive than those targeting single-copy genes and the additional use of a PCR targeting a single-copy gene can allow accurate quantification of the bacterial load present within a sample.
- Subjects
GERMANY; SWEDEN; UNITED Kingdom; DIAGNOSTIC services; POLYMERASE chain reaction; COXIELLA burnetii; NUCLEIC acids; GENES
- Publication
Journal of Veterinary Diagnostic Investigation, 2011, Vol 23, Issue 1, p108
- ISSN
1040-6387
- Publication type
Article
- DOI
10.1177/104063871102300118