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- Title
北方白菜型冬油菜BrFLC家族的全基因组鉴定、克隆及 表达分析.
- Authors
姚彦林; 马骊; 刘丽君; 李学才; 李鹏; 王旺田; 蒲媛媛; 牛早霞; 徐芳; 孙万仓; 武军艳
- Abstract
Vernalization is the main stage of bolting and flowering of winter plants, FLC (FLOWERING LO⁃ CUS C) integrates upstream signals to regulate flowering during vernalization. To understand the characteristics of FLC genes in winter rapeseeds (B. rapa) and its function in vernalization, 10 BrFLCs were identified from winter rapeseed genome using 6 Arabidopsis FLC genes as queries, 8 of which contained 7 exons. 10 BrFLCs are distribut⁃ ed on 4 chromosomes with obvious gene duplications. Evolutionary and conserved structure analysis showed that members in the same class were clustered with similar protein conserved motif distributions. The 1500 bp upstream promoter region of all the genes contained light response elements, among which BrFLC4, BrFLC5 and BrFLC6 con⁃ tained low temperature response elements, and BrFLC3, BrFLC4, BrFLC7 and BrFLC9 contained hormone response elements. BrFLC6 gene sequence located on A02, it was quite different from the annotated Bra031886 in B. rapa. Thus we consequently cloned the BrFLC6 genes from Longyou 6, Longyou 17, Tianyou 2, and Tianzhu small rape, and found that their sequence alignment similarity reached 99.5%. By analyse the BrFLC6 gene with the greatest se⁃ quence difference, qRT-PCR detection showed that could be expressed in roots, stems, leaves, flower buds and flow⁃ ers. BrFLC6 showed a down-regulation trend after vernalization, but the down-regulation extent was different among cultivars. Combined with the vernalization rate, it indicated that vernalization process might inhibited BrFLC6 expression, and its expression level was related to the time required by winter rapeseeds to pass through vernalization.
- Subjects
GENE expression; FLOWERING of plants; CHROMOSOME duplication; SEQUENCE alignment; VERNALIZATION
- Publication
Chinese Journal of Oil Crop Sciences, 2023, Vol 45, Issue 1, p83
- ISSN
1007-9084
- Publication type
Article
- DOI
10.19802/j.issn.1007-9084.2021314