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- Title
Functional analysis of the GRMZM2G174449 promoter to identify Rhizoctonia solani-inducible cis-elements in maize.
- Authors
Fangfang Yang; Xinhua Ding; Jing Chen; Yanting Shen; Lingguang Kong; Ning Li; Zhaohui Chu
- Abstract
Background: Banded leaf and sheath blight (BLSB), caused by the necrotrophic fungus Rhizoctonia solani, is a highly devastating disease in most maize and rice growing areas of the world. However, the molecular mechanisms of perceiving pathogen signals are poorly understood in hosts. Results: Here, we identified a Rhizoctonia solani-inducible promoter pGRMZM2G174449 in maize. Deletion analysis showed that the -574 to -455 fragment was necessary for pGRMZM2G174449 in responding to R. solani and this fragment contained the unknown pathogen-inducible cis-elements according to the bioinformatics analysis. Furthermore, detailed quantitative assays showed that two cis-elements, GCTGA in the -567 to -563 region and TATAT in the -485 to -481 region, were specifically responsible for the R. solani-inducible activity. A series of point mutation analysis indicated that the two cis-elements have the conserved motifs of NHWGN and DWYWT, respectively. Conclusion: Our results indicated that pGRMZM2G174449 is a good R. solani-inducible promoter suitable for genetic engineering of BLSB resistance. And NHWGN and DWYWT are two R. solani-inducible cis-elements that play important roles in pGRMZM2G174449 responding to R. solani.
- Subjects
CORN genetics; CORN research; RHIZOCTONIA solani; RHIZOCTONIA diseases; GENETIC mutation
- Publication
BMC Plant Biology, 2017, Vol 17, p1
- ISSN
1471-2229
- Publication type
Article
- DOI
10.1186/s12870-017-1181-5