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- Title
Effects of stimulating interleukin -2/anti- interleukin -2 antibody complexes on renal cell carcinoma.
- Authors
Kyu-Hyun Han; Ki Won Kim; Ji-Jing Yan; Jae-Ghi Lee; Eun Mi Lee; Miyeon Han; Eun Jin Cho; Seong Sik Kang; Hye Jin Lim; Tai Yeon Koo; Curie Ahn; Jaeseok Yang; Han, Kyu-Hyun; Kim, Ki Won; Yan, Ji-Jing; Lee, Jae-Ghi; Lee, Eun Mi; Han, Miyeon; Cho, Eun Jin; Kang, Seong Sik
- Abstract
<bold>Background: </bold>Current therapies for advanced renal cell carcinoma (RCC) have low cure rates or significant side effects. It has been reported that complexes composed of interleukin (IL)-2 and stimulating anti-IL-2 antibody (IL-2C) suppress malignant melanoma growth. We investigated whether it could have similar effects on RCC.<bold>Methods: </bold>A syngeneic RCC model was established by subcutaneously injecting RENCA cells into BALB/c mice, which were administered IL-2C or phosphate-buffered saline every other day for 4 weeks. RCC size was measured serially, and its weight was assessed 4 weeks after RENCA injection. Immune cell infiltration into RCC lesions and spleen was assessed by flow cytometry and immunohistochemistry.<bold>Results: </bold>IL-2C treatment increased the numbers of CD8(+) memory T and natural killer (NK) cells in healthy BALB/c mice (P < 0.01). In the spleen of RCC mice, IL-2C treatment also increased the number of CD8(+) memory T, NK cells, and macrophages as compared to PBS-treated controls (P < 0.01). The number of interferon-γ- and IL-10-producing splenocytes increased and decreased, respectively after 4 weeks in the IL-2C-treated mice (P < 0.01). Tumor-infiltrating immune cells including CD4(+) T, CD8(+) T, NK cells as well as macrophages were increased in IL-2C-treated mice than controls (P < 0.05). Pulmonary edema, the most serious side effect of IL-2 therapy, was not exacerbated by IL-2C treatment. However, IL-2C had insignificant inhibitory effect on RCC growth (P = 0.1756).<bold>Conclusions: </bold>IL-2C enhanced immune response without significant side effects; however, this activity was not sufficient to inhibit RCC growth in a syngeneic, murine model.
- Subjects
INTERLEUKIN-2; RENAL cell carcinoma; CD8 antigen; IMMUNE complexes; KILLER cells; TUMORS; IMMUNOHISTOCHEMISTRY; FLOW cytometry; ANIMAL experimentation; ANTIGENS; CELL lines; CELL physiology; KIDNEY tumors; MACROPHAGES; MICE; MONOCLONAL antibodies; SPLEEN; T cells
- Publication
BMC Urology, 2016, Vol 16, p1
- ISSN
1471-2490
- Publication type
journal article
- DOI
10.1186/s12894-016-0121-2