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- Title
Multiple DNA Transfer Events in a Social Setting Complicates Interpretation of DNA Evidence.
- Authors
Rizor, Leann G.; Stone, Jonah W. P.; Latham, Krista E.; Cale, Cynthia M.; Bush, Gay L.
- Abstract
Advances in forensic DNA typing and increases in the sensitivity of STR kits have allowed for the analysis of DNA transferred both directly and indirectly between individuals and objects. The present study was adapted from previous research and illustrates the transfer of genetic material between individuals and objects in a simulated social setting. Presterilized objects were handled by four participants sitting at a table. The order and timing of handling were predetermined and controlled by researchers to test for evidence of primary, secondary, and tertiary DNA transfer. Participant behavior and the actions of nonparticipants were not controlled by the researchers thereby mimicking a social situation. The handled objects and participants' hands were swabbed throughout the experiment. The DNA was purified, quantified, and amplified for DNA profiling. DNA was detected in 92% of the samples; however, only 50% produced profiles that met casework requirements for interpretation. Eighty percent of the interpretable DNA profiles were characterized as mixtures of DNA from two or more individuals, with 60% of those mixtures having identifiable major and minor contributors. Extraneous DNA was observed in 75% of the DNA profiles. Profiles obtained from the objects displayed no pattern regarding which participant most recently touched the object, and many of the samples were inconclusive because of the complexity of the mixtures. The open-air setting of this experiment and the extraneous DNA detected in the samples complicated reconstructing the order of transfer events. This study highlights the complexity of DNA transfer between individuals and objects when multiple transfer events occur.
- Subjects
DNA; DNA fingerprinting; DNA analysis
- Publication
Journal of Forensic Identification, 2020, Vol 70, Issue 2, p219
- ISSN
0895-173X
- Publication type
Article