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- Title
Occurrence of Groundnut ringspot virus on Brazilian peanut crops.
- Authors
Camelo-García, Viviana; Lima, Élison; Mansilla-Córdova, Pedro; Rezende, Jorge; Kitajima, Elliot; Barreto, Modesto
- Abstract
A high incidence of plants with mosaic, chlorotic spots, ringspots, necrosis, smaller leaves, and stunting was observed on peanut crops ( Arachis hypogaea L.) in Itápolis, São Paulo State, Brazil. Transmission electron microscope examination of thin sections of infected leaves revealed the presence of spheroidal particles, ca. 80 nm in diameter, suggestive of Tospovirus. A DNA fragment of ~600 bp was amplified by RT-PCR from total RNA extracted from infected tissues using primers specific for the nucleocapsid gene of Groundnut ringspot virus (GRSV). Nucleotide and deduced amino acid sequences of the fragments showed high identities with known GRSV isolates.
- Subjects
PEANUT diseases &; pests; MOSAIC diseases; VIRUS diseases of plants; CHLOROSIS (Plants); NUCLEOTIDES; FRANKLINIELLA; NECROSIS; REVERSE transcriptase polymerase chain reaction; PLANTS
- Publication
Journal of General Plant Pathology, 2014, Vol 80, Issue 3, p282
- ISSN
1345-2630
- Publication type
Article
- DOI
10.1007/s10327-014-0518-2