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- Title
Intralaboratory comparison of analytical methods for quantification of major phytocannabinoids.
- Authors
Béres, Tibor; Černochová, Lucie; Ćavar Zeljković, Sanja; Tarkowski, Petr; Benická, Sandra; Berčák, Michal; Gucký, Tomáš
- Abstract
This study compares alternative approaches for analyzing phytocannabinoids in different plant materials. Three chromatographic analytical methods (ultra-high-performance liquid chromatography with tandem mass spectrometric detection and gas chromatography with mass spectrometric and flame ionization detection) were evaluated regarding selectivity, sensitivity, analytical accuracy, and precision. The performance of the methods was compared and all three methods were demonstrated to be appropriate tools for analyzing phytocannabinoids in cannabis. Gas chromatography coupled with mass spectrometric detection showed slightly better accuracy in determining phytocannabinoid acids, which are often difficult to quantify owing to their limited stability. Aspects of sample preparation, such as material homogenization and extraction, were also considered. A single ultrasonic-assisted ethanolic extraction of dried and powdered plant samples of cannabis was shown to be exhaustive for extracting the samples prior to analysis.
- Subjects
CANNABINOIDS; CHROMATOGRAPHIC analysis; CANNABIS (Genus); LIQUID chromatography; GAS chromatography/Mass spectrometry (GC-MS); FLAME ionization detectors
- Publication
Analytical & Bioanalytical Chemistry, 2019, Vol 411, Issue 14, p3069
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-019-01760-y