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- Title
Minimal growth in vitro conservation of coffee ( Coffea spp.).
- Authors
Bertrand-Desbrunais, Anna; Noirot, Michel; Charrier, André
- Abstract
We have studied the influence of low concentrations of 6-benzyladenine on growth limitation, in order to preserve coffee germplasm through a microcutting collection. Concentrations of 0 μM, 1.3 μM and 4.4 μM were compared in four species: Coffea congensis, C. canephora, C. liberica and C. racemosa. After six months, microcutting behaviour varied between the different treatments, and a species effect was observed. The slow growing species ( C. liberica and C. congensis) needed 1.3 μM; the others coffee species ( C. canephora and C. racemosa) exhibited moderate caulogenesis on 6-benzyladenine-free medium. Zero and low concentrations did not affect survival rates. In conclusion 1.3 μM seems most appropriate for conserving all four species.
- Publication
Plant Cell, Tissue & Organ Culture, 1991, Vol 27, Issue 3, p333
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1007/BF00157599