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- Title
Production of a thermophilic maltooligosyl-trehalose synthase in Lactococcus lactis.
- Authors
Cimini, Donatella; De Rosa, Mario; Panariello, Andrea; Morelli, Veronica; Schiraldi, Chiara
- Abstract
The thermoacidophilic archaeon Sulfolobus solfataricus MT4 encodes a maltooligosyltrehalose synthase (MTS), that catalyzes an intramolecular transglycosylation process converting the glycosidic linkages at the reducing end of dextrins from α-1,4 into α-1,1. In this research the gene encoding MTS was cloned and expressed in Lactococcus lactis NZ9000 using the so-called NICE system. Growth conditions of the recombinant strain were optimized in flask experiments in relation to enzyme production. Batch experiments in 2 L-fermenters were performed on the best identified semidefined medium and 256 U L−1 of recombinant MTS were produced. Purified recombinant MTS shows its optimal activity at 70 °C and pH 5.5, prefers maltoheptaose and maltohexaose as substrates, and demonstrates minimal side hydrolytic activity.
- Subjects
STREPTOCOCCACEAE; GRAM-positive bacteria; LACTOCOCCUS; LACTOCOCCUS lactis; HYDROGEN-ion concentration; POLYBLEPHARIDACEAE; AMINO acid neurotransmitters; AMINO acids; SALT pans (Geology)
- Publication
Journal of Industrial Microbiology & Biotechnology, 2008, Vol 35, Issue 10, p1079
- ISSN
1367-5435
- Publication type
Article
- DOI
10.1007/s10295-008-0384-z