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- Title
斑蝥素类衍生物对粘虫胃毒活性 及解毒酶系的影响.
- Authors
席 娜; 孙 红; 孙文博; 郑胜礼; 张雅林
- Abstract
[Objectives] Screening from cantharidin derivatives to find high efficient, low toxic and residual chemicals for R&D of new pesticides. [Methods] Using bioassay and enzymatic determination methods to test 100 cantharidin derivatives. Stomach poison activity to the 3rd-instar larvae of Mythimna separata was tested by using leaf disc feeding method and their activity of the detoxification enzyme system was determined by sublethal dose. [Results] Bioassay results suggested that the effects of the derivatives Ⅰ-7# and Ⅳ-13# were better than norcantharidin; the stomach poison LC50 of Ⅰ-7# was similar to cantharidin and their LC50 were 49.818 and 43.677,g/mL respectively. The results of enzyme activity assay elucidated that cantharidin and its derivatives norcantharidin, Ⅰ-7# and Ⅳ-13# influenced detoxifying enzyme system of M. separata in different degrees. Among them, the specific activity of cytochrome P450 (P450) significantly reduced while carboxylesterase (CarE) is activated in different degrees by the cantharidin derivatives, and the specific activity of glutathione S-transferase (GST) generally showed activation at first and then inhibition. [Conclusion] Cantharidin derivativesⅠ-7# andⅣ-13# have strong insecticidal activity against lepidopteran pests and are of value for development and utilization as biopesticides.
- Subjects
MYTHIMNA separata; TOXICOLOGY of insecticides; PHYSIOLOGICAL effects of pesticides; BIOPESTICIDES; BIOLOGICAL assay; BIOTRANSFORMATION (Metabolism)
- Publication
Chinese Journal of Applied Entomology, 2018, Vol 55, Issue 3, p464
- ISSN
2095-1353
- Publication type
Article
- DOI
10.7679/j.issn.2095-1353.2018.059