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- Title
不同玉米芯含量对培养料中酶活和木耳产量及其子实体中氨基酸含量的影响
- Authors
陈辉; 王倩; 汪虹; 赵静; 郝海波; 隽加香; 陈明杰; 张津京
- Abstract
Effects of different concentrations of corncob on substrate ligninase and cellulase activities, fruiting body yield and amino acid contents of Auricularia auricula-judae were investigated.The corncob content in the substrate was 10%, 30%, 50% and 70% respectively. After the mycelia grew full of bags (mycelium stage) and fruiting body stage (4thflush), the substrates were sampled from the upper, middle and lower regions of the bags to determine enzyme activities, lignin content and cellulose content. The 4th flush of fruiting bodies were collected for amino acid analysis.It was found that the mycelium growth rate was the fastest when the corncob content in the substrate was 50%. In the mycelium stage, the lignin peroxidase activity was the highest when the corncob content in the substrate was 70%, the manganese peroxidase activity was the highest when the corncob content was 50%, and the laccase activity decreased with the increase of the corncob content. Carboxymethyl cellulase activity was high in all the 4 treatments, especially when the content of corncob was 30%.The activity of N-acetyl-β-glucosidase increased with the increase of corncob content. The activities of β-1,3-glucanase, β-glucosidase and endo-β-1,4-glucosidase increased first and then decreased, which peaked when the corncob content was 50%. In the fruiting body stage, the enzymes except lignin peroxidase and laccase increased with the increase of corncob content, which were different from the mycelium stage.In the mycelium stage,with the increase of corncob content, the lignin content in the substrate gradually decreased, and the cellulose content gradually increased.In the fruiting body stage, with the increase of corncob content, lignin and cellulose contents in the substrate decreased first and then increased, and the lowest contents were found when the corncob content in the substrate was 50%.When the content of corncob was 50%, the total unit yield per bag of fruiting body was the highest; when the content of corncob was 30%, the content of amino acids and their derivatives in fruiting body was the highest, including eight indispensable amino acids. Thus, the addition of corncob to the culture substrate improved the enzyme activities related to lignin and cellulose degradation, but the secretion patterns of different enzymes in substrates with different corncob contents were divergent.
- Subjects
AMINO acid derivatives; MANGANESE peroxidase; CORNCOBS; FRUIT yield; AMINO acids; LIGNINS; PEROXIDASE; GLUCOSIDASES
- Publication
Acta Edulis Fungi, 2019, Vol 26, Issue 2, p45
- ISSN
1005-9873
- Publication type
Article
- DOI
10.16488/j.cnki.1005-9873.2019.02.007