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- Title
Üriner Sistem Enfeksiyonlu Bireylerden İzole Edilen Escherichia coli ve Klebsiella pneumoniae Suşlarında CTX-M, TEM ve SHV Tipi Genişlemiş Spektrumlu Beta-laktamaz Aktivitesinin Otomatize Sistem ve Moleküler Yöntemlerle Araştırılması
- Authors
Deniz, Sibel; Büyük, Fatih; Murat, Kenan
- Abstract
Aim: This study aimed to investigate ESBL positivity among E.coli and K.pneumoniae strains isolated from urine samples belonging to individuals who applied to Kars Harakani State Hospital with urinary tract complaints by automated identification system and molecular methods. Material and Method: 5000 urine samples obtained between April and August 2019 were included. The samples were cultured on Mac Conkey and 7% sheep blood agar, identification was done by ID panel of BD Phoenix ™ 100 and antibiotic susceptibility and ESBL activities were determined by AST panel. CTX-M, TEM and SHV genes were analyzed by PCR. Results: 19.5% aerobic bacteria culture positivity was obtained from the urine samples and 120 (13.33%) of them were found to have ESBL activity. 102 (85%) of the isolates were identified as E.coli and 14 (11.67%) as K.pneumoniae. The ESBL positivity was obtained 2.4% for total urine samples, and 2.04% and 0.28% for E.coli and K.pneumoniae, respectively. While the total resistance to beta-lactam antibiotics was 57.46%, the most common resistance was observed against cephalosporins (92.53%) and the most common sensitivity (47.19%) was to carbapenems. At least one or more resistance genes were detected in 90.52% of ESBL positive isolates by PCR and CTX-M was the most common resistance gene. While there was no statistically significant relationship between ESBL positivity and age groups, however, a positive relationship was found between ESBL and gender of the patients at a rate of 21.9%. Conclusion: In addition to eliminating risk factors in reducing the incidence of ESBL-related infections, identification of microorganism, disclosure of relevant ESBL profiles and the right antibiotic preference to be developed according to these emerged models are important. It is hoped that this systematic study carried out in a limited location in Kars region of Turkey will benefit in this context.
- Subjects
PHOENIX (Ariz.); TURKEY; BETA lactam antibiotics; URINARY organs; AEROBIC bacteria; SYSTEM identification; GENDER; CARBAPENEMS
- Publication
Kafkas Journal of Medical Sciences / Kafkas Tıp Bilimleri Dergisi, 2021, Vol 11, Issue 2, p307
- ISSN
2146-2631
- Publication type
Article
- DOI
10.5505/kjms.2021.53367