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- Title
EFFECTS OF BIOPESTICIDES IN Tetragonisca angustula LATREILLE (HYMENOPTERA: MELIPONINAE) POLLINATORS.
- Authors
Prescinato de Oliveira, Carlos Vinício; Sinópolis Gigliolli, Adriana Aparecida; Galhardo, Douglas; Rodrigues Moreira, Daiani; Ronqui, Ludimilla; Aparecida Santos, Simone; Arnault Toledo, Vagner Alencar; Colla Ruvolo-Takasusuki, Maria Claudia
- Abstract
Stingless bees Tetragonisca angustula (Latreille) (Hymenoptera: Meliponinae) are pollinators of native and cultivated plants and are therefore in contact with areas contaminated by pesticides. These native bees were evaluated for changes in gene expression of esterase isoenzymes (EST) and peptides after contamination by contact with growth regulators from insecticides Gallaxy® EC 100, Natuneem and Azamax after 48, 120, 168 hours, 30 and 60 days. EST-4 presented an increase in relative activity after contamination with Gallaxy® EC 100 at 6.2 × 10-2 g a.i./mL; Natuneem at 7.5 × 10-5 g a.i./mL; and Azamax at 1.2 × 10-3 g a.i/mL after 60 days, 48 h, and 60 days, respectively. Inhibition of the relative activity of EST-4 was detected after contamination by Natuneem at 1.5 × 10-5 g a.i./mL and Azamax at 1.2 × 10-3 g a.i./mL after 48 h and 30 days, respectively. The insecticide growth regulators promoted changes in protein synthesis of T. angustula adult workers resulting in an increase or decrease in the relative intensity of bands, and the appearance of new peptides when compared with controls. Changes in protein synthesis have been identified mainly after long period of contamination, 120 and 168 h with the IGRs Gallaxy® EC 100 (at 0.78 and 1.25 g a.i./mL), Azamax (at 1.2 × 10-3 and 6 × 10-3 g a.i./mL), and Natuneem (at 7.5 × 10-5 and 3 × 10-3 g a.i./mL), and at 60 days with Natuneem (at 1.5 × 10-5 g a.i./mL). KEYWORDS: Biopesticide growth regulators. Esterases. Peptides. Stingless bees
- Publication
Arquivos de Ciências Veterinárias e Zoologia da Unipar, 2020, Vol 23, Issue 2, p1
- ISSN
1415-8167
- Publication type
Article
- DOI
10.25110/arqvet.v23i2cont.2020.8025