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- Title
Inhibitory effect of dietary monoglucosylceramide 1-O-β-glucosyl- N-2′-hydroxyarachidoyl-4,8-sphingadienine on two different categories of colon preneoplastic lesions induced by 1,2-dimethylhydrazine in F344 rats.
- Authors
Inamine, Morihiko; Suzui, Masumi; Morioka, Takamitsu; Kinjo, Tatsuya; Kaneshiro, Tatsuya; Sugishita, Tomoko; Okada, Tadashi; Yoshimi, Naoki
- Abstract
Sphingolipids display a wide spectrum of biological activities, including cell growth, differentiation and apoptosis. However, precise mechanisms by which these compounds exert anticancer or cancer-preventive effects are not known. In the present study, we evaluated the preventive efficacy of enriched dietary monoglucosylceramide 1-O-β-glucosyl- N-2′-hydroxyarachidoyl-4,8-sphingadienine (G1CM) on 1,2-dimethylhydrazine (DMH)-induced aberrant crypt foci (ACF) and β-catenin-accumulated crypt (BCAC) formation in F344 rats during initiation stage. We also examined whether G1CM affects cell proliferation and apoptosis in these lesions. Pure G1CM was isolated from rice bran. Forty-two rats were divided randomly into five experimental groups. Rats in groups 1–3 were given subcutaneous injections of DMH (40 mg/kg body weight) once a week for 2 weeks. One week before the first injection of DMH, rats in groups 2 and 3 were fed a diet containing 200 and 1000 p.p.m. G1CM, respectively, for 5 weeks. Rats in group 4 were fed a diet containing 1000 p.p.m. G1CM. Rats in group 5 were given the basal diet alone and served as untreated controls. The experiment was terminated 5 weeks after the start. Dietary G1CM at both doses (groups 2 and 3) significantly inhibited the induction of ACF and BCAC ( P < 0.001) when compared to group 1 treated with DMH alone. In groups 2 and 3, the proliferating cell nuclear antigen labeling indices of epithelial cells in ACF and BCAC were also lower than in group 1 ( P < 0.0001 for ACF, P < 0.05 for BCAC). These results, that dietary G1CM has possible chemopreventive effects in the present short-term colon carcinogenesis bioassays, suggest that longer exposure may cause suppression of tumor development. ( Cancer Sci 2005; 96: 876–881)
- Subjects
APOPTOSIS; CELL death; CANCER; SPHINGOLIPIDS; SPHINGOSINE; CELL cycle; CELL populations; CELL proliferation; CELL growth
- Publication
Cancer Science, 2005, Vol 96, Issue 12, p876
- ISSN
1347-9032
- Publication type
Article
- DOI
10.1111/j.1349-7006.2005.00127.x