We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Ultrasound-Assisted Extraction of Bioactive Compounds from Annatto Seeds, Evaluation of Their Antimicrobial and Antioxidant Activity, and Identification of Main Compounds by LC/ESI-MS Analysis.
- Authors
Quintero Quiroz, Julian; Naranjo Duran, Ana Maria; Silva Garcia, Mariluz; Ciro Gomez, Gelmy Luz; Rojas Camargo, John Jairo
- Abstract
This study evaluated the antimicrobial activity (i.e., against Bacillus cereus and Staphylococcus aureus) and the antioxidant activity (i.e., ABTS, FRAP, and DPPH) of annatto seeds extract obtained by ultrasound-assisted extraction. A response surface design with three levels such as pH (2-11), solvent concentration (50-96 %), seed-to-solvent ratio (1:2–1:10), and treatment time (0-30 min) was employed to determine the optimal experimental conditions. Thus, a pH of 7.0, seed-to-solvent ratio of 1:7, and treatment time of 20 min were selected as optimal rendering an extract having a 0.62% of bixin, 3.81 mg gallic acid/mg equivalent of polyphenol compounds (ABTS 1035.7, FRAP 424.7, and DPPH 1161.5 μM trolox/L), and a minimal inhibitory concentration against Bacillus cereus and Staphylococcus aureus of 32 and 16 mg/L, respectively. Further, the main bioactive compounds identified by LC/ESI-MS were bixin and catechin, chlorogenic acid, chrysin, butein, hypolaetin, licochalcone A, and xanthohumol.
- Subjects
ACHIOTE; BIOACTIVE compounds; ANTI-infective agents; MASS spectrometry; EXTRACTION techniques
- Publication
International Journal of Food Science, 2019, p1
- ISSN
2356-7015
- Publication type
Article
- DOI
10.1155/2019/3721828