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- Title
Next-generation sequencing technology in the diagnosis of five patients with human herpesvirus encephalitis/meningitis.
- Authors
JIN Ke; WANG Xiao-juan; GUAN Hong-zhi; QIN Ling-zhi; JIA Ya-zhen; ZHOU Ke-ke; MA Hai-chang; WANG Yue; WANG Fang; XING Li; LI Wei
- Abstract
Objective To evaluate the value of next-generation sequencing (NGS) of cerebrospinal fluid (CSF) in the diagnosis of human herpesvirus encephalitis/meningitis. Methods The clinical features, laboratory and imaging findings of 5 patients with highly suspected human herpesvirus encephalitis/ meningitis from January to September 2018 were retrospectively analyzed, and then the etiological sources were comprehensively analyzed combined with the results of NGS. Results All of the 5 patients had an acute onset, fever, headache and neck ankylosis. The CSF tests revealed a slight increased level of white blood cell count (74-260) x 106/L. Among them, a total of 4-5406 nucleic acid sequences of human herpesvirus, including human herpesvirus type I (n = 1), human herpesvirus type III (n = 3) and human herpesvirus type IV (n = 1) were detected by NGS, with the coverage varing from 0.08% to 81.01% . Conclusions NGS of CSF can assist the diagnosis of human herpesvirus encephalitis/meningitis, as well as viruses accurate classification, which is worth popularizing and applying in clinic.
- Subjects
CHINA; CEREBROSPINAL fluid examination; HERPESVIRUS disease genetics; DNA; HERPESVIRUS diseases; RETROSPECTIVE studies; LEUKOCYTE count; VIRAL encephalitis; VIRAL meningitis; SEQUENCE analysis
- Publication
Chinese Journal of Contemporary Neurology & Neurosurgery, 2020, Vol 20, Issue 7, p638
- ISSN
1672-6731
- Publication type
Article
- DOI
10.3969/j.issn.1672-6731.2020.07.014