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- Title
The novel high molecular weight Dermatophagoides farinae protein Zen-1 is a major allergen in North American and European mite allergic dogs with atopic dermatitis.
- Authors
Olivry, Thierry; Dunston, Stanley M.; Favrot, Claude; Prélaud, Pascal; Tsukui, Toshiroh
- Abstract
Background Atopic dogs with hypersensitivity to Dermatophagoides farinae (Df) have IgE recognizing high molecular weight ( MW) allergens more often than the low MW Der f 1 and 2. A new high MW Df allergen, Zen-1, has been identified recently. Objectives To determine the IgE reactivity of American and European Df-hypersensitive dogs to Zen-1, Der f 1 and Der f 2. Methods We tested sera from 33 Df-reactive dogs from the USA, 29 from Europe and 15 experimentally sensitized to Df, by ELISA against crude Df, Der f 1, Der f 2 and Zen-1. ELISA inhibition was performed with sera reactive to Zen-1. Intradermal testing ( IDT) was also done with the same allergens in 25 other American atopic dogs. Results Altogether, IgE seropositivity to Zen-1 was more prevalent (86%) than that to Der f 1 (17%) or Der f 2 (19%). The IgE reactivity to Zen-1 was correlated to that against crude Df; this allergen alone inhibited a high percentage (median: 50%; range: 22-84%) of the binding to the crude mite extract. The seropositivity to low MW allergens was highest in experimentally sensitized dogs. Serum IgE recognition of Der f 1 was low in dogs with AD; that to Der f 2 was significantly lower in American dogs (6%) than in European ones (28%). A high prevalence of positive immediate IDT reactions to Zen-1 confirmed the likely relevance of serological results. Conclusions and clinical importance This study establishes Zen-1 as a major allergen in atopic dogs sensitized to Df.
- Subjects
DERMATOPHAGOIDES; ALLERGENS; ATOPIC dermatitis; MITES; DOG diseases; IMMUNOGLOBULIN E
- Publication
Veterinary Dermatology, 2017, Vol 28, Issue 2, p177
- ISSN
0959-4493
- Publication type
Article
- DOI
10.1111/vde.12393