We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
QUANTITATIVE ULTRASTRUCTURAL VARIATIONS BETWEEN PITYROSPORUM OVALE AND P. ORBICULARE BASED ON SERIAL SECTION ELECTRON MICROSCOPY.
- Authors
Keddie, F. M.; Barajas, L.
- Abstract
From serial section electron micrographs, the ultrastructural cytology of whole cells of Pityrosporum ovale and P. orbiculare was analyzed volumetrically and illustrated in three-dimensional images. Cells cultured on sodium taurocholate medium were fixed in both glutaraldehyde and potassium permanganate solutions and imbedded in Vestopal for serial sectioning. The volume of the cell, mitochondria and nucleus was obtained by measurements of successive sections through whole cells. The sections of one cell were built in a solid model. The average number of mitochondria in the cells of P. ovale was 23 and in P. orbiculare 2.6 per cell. The volume of the mitochondria was from 14% to 37% of the cell volume. The mitochondria of P. orbiculare appear to increase in size with cell volume, whereas in P. ovale, there may be an increase in number rather than in size. Two types of cellular bodies, one electron light and one electron dense, present in each species, also showed differences in size and number. The volume of the nucleus was 6 to 9% of the cell volume. The serial section analysis points to differences in mitochondrial biogenesis which may be of taxonomic value in separating the two species of Pityrosporum.
- Subjects
ELECTRON microscopy; MALASSEZIA ovale; POTASSIUM permanganate; CYTOLOGY; MICROTOMY
- Publication
International Journal of Dermatology, 1972, Vol 11, Issue 1, p40
- ISSN
0011-9059
- Publication type
Article
- DOI
10.1111/j.1365-4362.1972.tb01720.x