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- Title
Biogenic Silver Nanoparticles by Pseudomonas aeruginosa Reduce Expression of Biofilm and Quorum Signaling Genes in Multi-drug Resistant Acinetobacter baumannii.
- Authors
Hasan, Talar Ibrahim; Ahmed, Akhter Ahmed
- Abstract
Discovery of antibiotics is regarded as one of the critical moments in the history of medicine; however, irrational use caused the emergence of a phenomenon known as drug resistance. Once considered benign, Acinetobacter baumannii (A. baumannii) evolved to become a challenging pathogen threatening the current antibiotic era. In 2017, WHO appointed A. baumannii as the most critical pathogen towards which the development of novel antibiotics is keenly required. The current study intends to explore the silver nanoparticles (AgNPs) role in the weakening of virulence and biofilm through reducing the expression of outer membrane protein-A (OmpA), biofilm-associated protein (Bap), Acinetobacter baumannii autoinducer-I (abaI) and Acinetobacter baumannii receptor (abaR) genes in the multi-drug resistant (MDR) A. baumannii. All bacterial isolates were capable to form biofilm and exhibited high resistance levels to the antibiotics used including (ampicillin/sulbactam, ceftazidime, tobramycin, amikacin, gentamicin, levofloxacin, imipenem, ciprofloxacin, meropenem, piperacillin/tazobactam, cefepime, ceftriaxone, doxycycline, and trimethoprim/sulfamethoxazole). AgNPs were biologically synthesized by Pseudomonas aeruginosa (P. aeruginosa) (PA-AgNP) and characterized via FTIR, UV-vis, EDX, XRD, and SEM. Results of characterization tools supported the successful formation of crystalline AgNPs. Minimum-inhibitory concentrations of the harvested AgNPs were determined to study their antibiofilm and quorum quenching potential at subinhibitory concentrations (SIC). RT PCR was utilized to estimate the influence of PA-AgNPs on the quorum sensing (QS) and biofilm at level of gene expression. Exposure of the tested isolates to PA-AgNP at SIC values decreased their biofilm fabrication capacity and significantly downregulated candidate genes expression. The results show that P. aeruginosa can be used to bio-fabricate AgNPs capable of interrupting bacterial-growth and biofilm progress in the MDR A. baumannii through the downregulation of QS and biofilm-associated genes.
- Subjects
ACINETOBACTER baumannii; GENE expression; SILVER nanoparticles; PSEUDOMONAS aeruginosa; BIOFILMS; CARIOGENIC agents; CARBAPENEMS; BIOGENIC amines; BENZOPYRENE
- Publication
Jordan Journal of Biological Sciences, 2023, Vol 16, Issue 4, p621
- ISSN
1995-6673
- Publication type
Article
- DOI
10.54319/jjbs/160407