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- Title
Widely Used Mutants of eiger, Encoding the Drosophila Tumor Necrosis Factor, Carry Additional Mutations in the NimrodC1 Phagocytosis Receptor.
- Authors
Kodra, Albana; de la Cova, Claire; Gerhold, Abigail R.; Johnston, Laura A.
- Abstract
The process of apoptosis in epithelia involves activation of caspases, delamination of cells, and degradation of cellular components. Corpses and cellular debris are then rapidly cleared from the tissue by phagocytic blood cells. In studies of the Drosophila TNF, Eiger (Egr) and cell death in wing imaginal discs, the epithelial primordia of fly wings, we noticed that dying cells appeared to transiently accumulate in egr³ mutant wing discs, raising the possibility that their phagocytic engulfment by hemocytes was impaired. Further investigation revealed that lymph glands and circulating hemocytes from egr³ mutant larvae were completely devoid of NimC1 staining, a marker of phagocytic hemocytes. Genome sequencing uncovered mutations in the NimC1 coding region that are predicted to truncate the NimC1 protein before its transmembrane domain, and provide an explanation for the lack of NimC staining. The work that we report here demonstrates the presence of these NimC1 mutations in the widely used egr³ mutant, its sister allele, egr1, and its parental strain, Regg1GS9830. As the egr³ and egr1 alleles have been used in numerous studies of immunity and cell death, it may be advisable to re-evaluate their associated phenotypes.
- Subjects
CELL anatomy; BLOOD cells; DROSOPHILA; CELL death; IMAGINAL disks; TUMOR necrosis factors; PHAGOCYTOSIS
- Publication
G3: Genes | Genomes | Genetics, 2020, Vol 10, Issue 12, p4707
- ISSN
2160-1836
- Publication type
Article
- DOI
10.1534/g3.120.401800