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- Title
Mass spectrometric analysis of combinatorial peptide libraries derived from the tandem repeat unit of MUC2 mucin.
- Authors
Gitta Schlosser; Zoltán Takáts; Károly Vékey; Gabriella Pócsfalvi; Antonio Malorni; Emöke Windberg; Andrea Kiss; Ferenc Hudecz
- Abstract
Four 19-member synthetic peptide libraries, based on the TX1TX2T epitope motif of the mucin-2 gastrointestinal glycoprotein (MUC2) and ranging in peptide length from dipeptides to 15-mers (XT, TXT, TQTXT and KVTPTPTPTGTQTXT), were synthesized by combinatorial solid phase peptide synthesis using the portioning-mixing combinatorial approach, and analysed by electrospray ionization mass spectrometry at different (100010 000) resolutions. Most of the components of the individual libraries could be easily identified in a single-stage molecular mass screening experiment. The resolving power of the instrument becomes an important factor above 8001000 Da molecular mass, when predominantly multiply charged molecular ions are formed. Approaches to the identification of isobars (glutamine/lysine), isomers (leucine/isoleucine) and sequence variations by tandem mass spectrometry, and/or by high-performance liquid chromatography-mass spectrometry are outlined. Copyright © 2003 European Peptide Society and John Wiley & Sons, Ltd.
- Subjects
PEPTIDE synthesis; ELECTROSPRAY ionization mass spectrometry; MUCINS; EPITOPES; GLYCOPROTEINS; HIGH performance liquid chromatography
- Publication
Journal of Peptide Science, 2003, Vol 9, Issue 6, p361
- ISSN
1075-2617
- Publication type
Article
- DOI
10.1002/psc.462