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- Title
Single Nucleotide Polymorphism-Based Haplotypes Associated with Charcoal Rot Resistance in Brazilian Soybean Germplasm.
- Authors
Vinholes, Patrícia; Rosado, Renato; Roberts, Philip; Borém, Aluízio; Schuster, Ivan
- Abstract
Incorporation of resistance to Charcoal Rot (CR), caused by the soil-borne fungus Macrophomina phaseolina (Tassi) Goid., is an important goal for soybean [Glycine max (L.) Merr.] breeding programs. However, the limited accessible information on sources and hereditary mechanisms related to resistance has impeded progress, as reflected in the lack of commercial CR-resistant soybean varieties around the world. The identification of single nucleotide polymorphisms (SNPs) and haplotypes associated with CR resistance will be an important breeding component. In this study, a genome wide association study (GWAS) was undertaken to identify genomic regions controlling CR resistance in a soybean association mapping panel, using single nucleotide polymorphism (SNP) markers and haplotype information. The soybean varieties (N = 169) were field-evaluated on a site with a history of heavy CR infestation in Citrus Research Center and Agricultural Experiment Station (CRC-AES) at the University of California, Riverside, USA, and genotyped with 6000 SNP markers. Symptoms of mortality were evaluated for 12 wk after the first symptoms of infection. Two SNP-based haplotypes were found associated with CR in the genome-wide haplotype association analysis. Two associations of stable haplotypes for CR, haplotype TAC and CGA of the markers Gm08_18909193_A_G, Gm08_44422211_T_C, and Gm19_34320762_A_C were identified. These markers are associated with other important disease resistance genes in the soybean genome. These findings will allow breeders to improve tropical soybean breeding for CR resistance. A haplotype-based GWAS can provide more information into the genetic basis of CR resistance that could not be captured by the single marker approach.
- Subjects
HAPLOTYPES; CHARCOAL rot; GERMPLASM
- Publication
Agronomy Journal, 2019, Vol 111, Issue 1, p182
- ISSN
0002-1962
- Publication type
Article
- DOI
10.2134/agronj2018.07.0429