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- Title
Pyruvate neutralizes peritoneal dialysate cytotoxicity: Maintained integrity and proliferation of cultured human mesothelial cells.
- Authors
Brunkhorst, Reinhard; Mahiout, Arezki
- Abstract
Toxic effects of commercially available peritoneal dialysate (PD) fluid include damage to mesothelial cells (MC), causing a severely disturbed proliferation of cultured MC. We investigated the injury to the cell membrane (by release of lactate dehydrogenase, LDH), the proliferation (by cell counts and by 3H-thymidine incorporation), and optional the cytokine generation (by IL-1 receptor-antagonist production, IL-1 ra) of cultured human MC during the 48 hours after a 30 minute exposure to PD containing either 35 mmol/liter sodium lactate or sodium pyruvate. All solutions had a pH of 5.2 to 5.6 and were composed as standard PD. Glucose contents of 1.36 and 3.86 mmol/liter were tested. After exposure to the lactate-PD containing 1.36% glucose, LDH activity was increased by more than 30%, proliferation of MC was inhibited by more than 30%, and IL-1 ra production was reduced significantly when compared to pyruvate-PD and the control solution. After preincubation with 3.86% glucose containing PD. all negative effects became even more pronounced in the lactate group whereas the MC maintained their integrity, rate of proliferation and IL-1 ra release after pre-exposure to pyruvate containing PD. These results suggest that the acute toxic effects of commercially available PD on the integrity, proliferation and IL-1 ra production of MC can be avoided by the use of sodium pyruvate instead of sodium lactate.
- Subjects
PYRUVATES; PYRUVIC acid; PERITONEAL dialysis; HEMODIALYSIS; CELLS; CELL membranes; LACTATE dehydrogenase; NEPHROLOGY
- Publication
Kidney International, 1995, Vol 48, Issue 1, p177
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.1995.282