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- Title
Effects of a low-fat diet on the hepatic expression of adiponectin and its receptors in rats with NAFLD.
- Authors
Hong Ma; Guo-Ping You; Fan Cui; Lu-Fang Chen; Xiang-Jiu Yang; Li-Gang Chen; Hua-Dong Lu; Wen-Qiang Zhang
- Abstract
Background. Non-alcoholic fatty liver disease (NAFLD) is correlated with obesity, but specific therapeutic interventions are lacking. Adiponectin is an adipokine with anti-inflammatory activity and is considered a hepatic protector. We aimed to investigate effects of a low-fat diet on the hepatic expression of adiponectin and its receptors in rats with NAFLD. Materials and methods. Sixteen male SD rats were fed a high-fat diet for 8 weeks (HFD1 group) or 16 weeks (HFD2 group) to induce NAFLD, and these rats were compared with rats on a normal diet for 8 weeks (NC1 group) or 16 weeks (NC2 group). Another group of 8 rats was fed an HFD for 8 weeks and then switched to a low-fat diet (DIET group) until the 16th week. The expression of hepatic adiponectin and its receptors was detected by western blotting, immunohistochemistry and RT-qPCR. Results. The NAFLD activity score (NAS) in the HFD groups increased from 3.2 ± 0.45 (8th week) to 6.2 ± 0.84 (16th week) (P < 0.001), reflecting the progression in the NAFLD histology. In contrast to the HFD2 group, the low-fat diet ameliorated the steatosis, ballooning degeneration and inflammation. Dietary intervention augmented the expression of adiponectin and its receptors, which was down-regulated in the HFD2 group. Conclusions. The NAFLD rat model was successfully developed by feeding the animals a high-fat diet. Adiponectin may play a role in the pathogenesis of NAFLD, especially in the progression from steatosis to NASH. The low-fat diet alleviated the histological lesions associated with NAFLD by up-regulating the expression of adiponectin and its receptors.
- Subjects
FATTY liver; LOW-fat diet; ADIPONECTIN; WESTERN immunoblotting; IMMUNOHISTOCHEMISTRY; FATTY degeneration; INFLAMMATION
- Publication
Annals of Hepatology: Official Journal of the Mexican Association of Hepatology, 2015, Vol 14, Issue 1, p108
- ISSN
1665-2681
- Publication type
Article
- DOI
10.1016/s1665-2681(19)30807-5