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- Title
Assembly of the Light-Harvesting Chlorophyll Antenna in the Green Alga Chlamydomonas reinhardtii Requires Expression of the TLA2-CpFTSY Gene.
- Authors
Kirst, Henning; Garcia-Cerdán, Jose Gines; Zurbriggen, Andreas; Melis, Anastasios
- Abstract
The truncated light-harvesting antenna2 (tla2) mutant of Chlamydomonas reinhardtii showed a lighter-green phenotype, had a lower chlorophyll (Chl) per-cell content, and higher Ch1 a/b ratio than corresponding wild-type strains. Physiological analyses revealed a higher intensity for the saturation of photosynthesis and greater Pmax values in the tla2 mutant than in the wild type. Biochemical analyses showed that the th12 strain was deficient in the Ch1 a-b light-harvesting complex, and had a Ch1 antenna size of the photosystems that was only about 65% of that in the wild type. Molecular and genetic analyses showed a single plasmid insertion in the tla2 strain, causing a chromosomal DNA rearrangement and deletion/disruption of five nuclear genes. The TLA2 gene, causing the tla2 phenotype, was cloned by mapping the insertion site and upon complementation with each of the genes that were deleted. Successful complementation was achieved with the C. reinhardtii TLA2-CpFTSY gene, whose occurrence and function in green microalgae has not hitherto been investigated. Functional analysis showed that the nuclear-encoded and chloroplast-localized CrCpFTSY protein specifically operates in the assembly of the peripheral components of the Ch1 a-b light-harvesting antenna. In higher plants, a cpftsy null mutation inhibits assembly of both the light-harvesting complex and photosystem complexes, thus resulting in a seedling-lethal phenotype. The work shows that cpftsy deletion in green algae, but not in higher plants, can be employed to generate th1 mutants. The latter exhibit improved solar energy conversion efficiency and photosynthetic productivity under mass culture and bright sunlight conditions.
- Subjects
CHLAMYDOMONAS reinhardtii; CHLOROPHYLL; GENE expression in plants; PHOTOSYNTHESIS genetics; PLANT gene mapping
- Publication
Plant Physiology, 2012, Vol 158, Issue 2, p930
- ISSN
0032-0889
- Publication type
Article
- DOI
10.1104/pp.111.189910