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- Title
Morphological Characterization and Species Verification Using Cytochrome C Oxidase Subunit I (COI) of Two Laguna Populations of the Cacao Pachyrhynchine Beetle, Pachyrhynchus moniliferus Germar, 1823 (Coleoptera: Curculionidae).
- Authors
Domingo, Kimberly Ann S.; Caoili, Barbara L.; Yap, Sheryl A.
- Abstract
Pachyrhynchus moniliferus Germar is one of the major insect pests in cacao plantations in Luzon Island, Philippines. It is morphologically very similar to other Pachyrhynchus species and, thus, often mistakenly identified. Consequently, inappropriate control measures are applied. Herein, two P. moniliferus populations from Laguna province, one from Los Baños and the other from Magdalena (Philippines), were morphologically examined and DNA barcoded. The Magdalena population has a bigger body size, as well as a longer and wider rostrum compared to the Los Baños population. Comparison between the Folmer fragment of the COI gene of the Pachyrhynchus from Los Baños and Magdalena to the nucleotide sequences in the GenBank revealed a best match of only 87% identity to P. speciosus Waterhouse (KU953498.1) and 88% to P. orbifer Waterhouse (KU953440.1), respectively. The COI sequence of P. moniliferus presented here is the first DNA barcode reported for this species (GenBank Accession Numbers: MT038197.2-MT038204.2). We recommend conducting more thorough morphometric studies in conjunction with genetic diversity analyses on the different P. moniliferus populations across the Philippine islands.
- Subjects
LUZON (Philippines); CYTOCHROME oxidase; CACAO; CURCULIONIDAE; NUCLEOTIDE sequence; SPECIES; CACAO beans
- Publication
Philippine Journal of Science, 2023, Vol 152, Issue 1, p351
- ISSN
0031-7683
- Publication type
Article
- DOI
10.56899/152.01.27