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- Title
Effect of vitamin E-bonded membrane on the 8-hydroxy 2′-deoxyguanosine level in leukocyte DNA of hemodialysis patients.
- Authors
Tarng, Der-Cherng; Huang, Tung-Po; Liu, Tsung-Yun; Chen, Haw-Wen; Sung, Yen-Jen; Wei, Yau-Huei
- Abstract
Effect of vitamin E-bonded membrane on the 8-hydroxy 2′-deoxyguanosine level in leukocyte DNA of hemodialysis patients. Background. 8-Hydroxy 2′-deoxyguanosine (8-OHdG) of leukocyte DNA has been identified as a surrogate marker of oxidative stress in chronic hemodialysis (HD) patients. In this study, we focused on the determinants of the 8-OHdG level in leukocyte DNA of HD patients. We further investigated the influence of vitamin E-modified, regenerated cellulose (CL-E) membrane on the oxidative DNA damage, intracellular reactive oxygen species (ROS) production of granulocytes, and plasma α-tocopherol concentration. Methods. 8-OHdG content in cellular DNA of leukocytes was measured by a high-performance liquid chromatography-electrochemical detection (HPLC-ECD) method. Intracellular production of ROS, H2 O2 and O2-· were analyzed by flow cytometry in leukocytes with and without phorbol-12-myristate-13-acetate (PMA) stimulation before dialysis, as well as at 15 and 30 minutes of dialysis. Plasma α-tocopherol concentration was measured by a HPLC method, and the value of α-tocopherol was corrected by total blood lipid concentration. Results. In the prospective cross sectional study, the mean 8-OHdG level in leukocyte DNA was equally lower in the patients of the CL-E, polymethylmethacrylate (PMMA), and polysulfone (PS) groups as compared with the cellulosic group (ANOVA, P < 0.001). The leukocyte 8-OHdG level correlated negatively with plasma α-tocopherol and blood lipid-adjusted plasma α-tocopherol, but correlated positively with serum iron and percentage of transferrin saturation. Forward stepwise multiple regression showed that dialysis membrane type, serum iron, and blood lipid-adjusted plasma α-tocopherol were the independent determinants of the leukocyte 8-OHdG level in HD patients. Like synthetic membranes, granulocyte ROS production was less augmented during...
- Subjects
HEMODIALYSIS; VITAMIN E; BIOAVAILABILITY
- Publication
Kidney International, 2000, Vol 58, Issue 2, p790
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1046/j.1523-1755.2000.00228.x