We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Humanization of autoantigen.
- Authors
Nishie, Wataru; Sawamura, Daisuke; Goto, Maki; Ito, Kei; Shibaki, Akihiko; McMillan, James R; Sakai, Kaori; Nakamura, Hideki; Olasz, Edit; Yancey, Kim B; Akiyama, Masashi; Shimizu, Hiroshi
- Abstract
Transmissibility of characteristic lesions to experimental animals may help us understand the pathomechanism of human autoimmune disease. Here we show that human autoimmune disease can be reproduced using genetically engineered model mice. Bullous pemphigoid (BP) is the most common serious autoimmune blistering skin disease, with a considerable body of indirect evidence indicating that the underlying autoantigen is collagen XVII (COL17). Passive transfer of human BP autoantibodies into mice does not induce skin lesions, probably because of differences between humans and mice in the amino acid sequence of the COL17 pathogenic epitope. We injected human BP autoantibody into Col17-knockout mice rescued by the human ortholog. This resulted in BP-like skin lesions and a human disease phenotype. Humanization of autoantigens is a new approach to the study of human autoimmune diseases.
- Subjects
BLISTERS; AMINO acid sequence; INFECTIOUS disease transmission; AUTOANTIBODIES; ANIMAL disease models; AUTOIMMUNE diseases
- Publication
Nature Medicine, 2007, Vol 13, Issue 3, p378
- ISSN
1078-8956
- Publication type
Article
- DOI
10.1038/nm1496