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- Title
Endogenous Galectin-3 Is Localized in Membrane Lipid Rafts and Regulates Migration of Dendritic Cells.
- Authors
Hsu, Daniel K.; Chernyavsky, Alexander I.; Chen, Huan-Yuan; Yu, Lan; Grando, Sergei A.; Liu, Fu-Tong
- Abstract
This study reveals a function of endogenous galectin-3, an animal lectin recognizing β-galactosides, in regulating dendritic cell motility both in vitro and in vivo, which to our knowledge is unreported. First, galectin-3-deficient (gal3−/−) bone marrow-derived dendritic cells exhibited defective chemotaxis compared to gal3+/+ cells. Second, cutaneous dendritic cells in gal3−/− mice displayed reduced migration to draining lymph nodes upon hapten stimulation compared to gal3+/+ mice. Moreover, gal3−/− mice were impaired in the development of contact hypersensitivity relative to gal3+/+ mice in response to a hapten, a process in which dendritic cell trafficking to lymph nodes is critical. In addition, defective signaling was detected in gal3−/− cells upon chemokine receptor activation. By immunofluorescence microscopy, we observed that galectin-3 is localized in membrane ruffles and lamellipodia in stimulated dendritic cells and macrophages. Furthermore, galectin-3 was enriched in lipid raft domains under these conditions. Finally, we determined that ruffles on gal3−/− cells contained structures with lower complexity compared to gal3+/+ cells. In view of the participation of membrane ruffles in signal transduction and cell motility, we conclude that galectin-3 regulates cell migration by functioning at these structures.Journal of Investigative Dermatology (2009) 129, 573–583; doi:10.1038/jid.2008.276; published online 9 October 2008
- Subjects
LECTINS; DENDRITIC cells; CELL motility; CHEMOTAXIS; HAPTENS; LYMPH node diseases; FLUORESCENCE microscopy
- Publication
Journal of Investigative Dermatology, 2009, Vol 129, Issue 3, p573
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1038/jid.2008.276