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- Title
Investigation of the presence of HPV on KTP laser fibers following KTP laser treatment of papilloma.
- Authors
Dodhia, Sonam; Baxter, Peter C.; Ye, Fei; Pitman, Michael J.
- Abstract
<bold>Objectives: </bold>Recurrent respiratory papillomatosis is often treated with in-office laser procedures using a potassium titanyl phosphate (KTP) laser transmitted through a laser fiber. Although effective, this procedure has notable downsides, including the possibility of transmitting human papillomavirus (HPV) in the smoke plume and the high cost of these single-use fibers. The objective of this study is to determine if HPV can be detected on a laser fiber after use, with or without sterilization.<bold>Methods: </bold>Twelve patients with laryngeal papillomas were treated with KTP laser energy transmitted via a KTP fiber. Ten fibers were sterilized in CIDEX (ASP, Irvine, California), a glutaraldehyde disinfectant, for 12 minutes, whereas two fibers were left unsterilized. Human papillomavirus DNA amplification was done on all 12 fiber samples with real-time polymerase chain reaction (PCR) using general primer mediated 5+ and 6+. Human papillomavirus genotyping detection was done using type specific probes and/or Sanger sequencing.<bold>Results: </bold>Over 27 strains of HPV were not detected on KTP fibers after use, with or without sterilization.<bold>Conclusion: </bold>Human papillomavirus was undetectable by PCR on KTP laser fibers that were sterilized or unsterilized after use. Further studies are needed utilizing a transmission model to determine if HPV can be incubated from this fiber after sterilization.<bold>Level Of Evidence: </bold>NA. Laryngoscope, 128:926-928, 2018.
- Subjects
POTASSIUM titanyl phosphate; PAPILLOMAVIRUSES; GENE amplification; POLYMERASE chain reaction; STERILIZATION (Disinfection); TREATMENT of respiratory diseases; PAPILLOMA; DISINFECTION &; disinfectants; LARYNX; MEDICAL lasers; SEQUENCE analysis; GENOTYPES; THERAPEUTICS
- Publication
Laryngoscope, 2018, Vol 128, Issue 4, p926
- ISSN
0023-852X
- Publication type
journal article
- DOI
10.1002/lary.27018