We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Epiregulin, a novel EGF receptor legend, promotes regeneration of renal epithelial cells.
- Authors
Shougang Zhuang; Yan Yan; Daubert, Rebecca A.; Schnellmann, Rick G.
- Abstract
Epiregulin is an epidermal growth factor (EGF) member that activates ErBB1 and ErBB4 homodimers and all possible herodimeric ErbB complex. However, its potential role in renal regeneration has not been investigated. In this study, we assessed the effect of exogenous epiregulin on renal epithelial cell regeneration in the primary culture of renal proximal tubular cells. We demonstrated that epiregulin had comparable effects with EGF in enhancing RPTC outgrowth, scattering, proliferation and migration. Epiregulin induced activation of the EGF receptor, Akt, a downstream kinase of phosphoinositide 3-kinase (P13K), and extracellular signaling-regulated kinase ½ (ERK1/2). Treatment with AG1478, a specific EGFR inhibitor, blocked phosphorylation of EGFR, Akt and ERK1/2. Further, inactivation of P13K with LY294002 blocked epiregulin-induced RPTC proliferation and to less extent, migration. However, blockade of ERK1/2 had no such effects. Therefore, these data suggest that epiregulin is a potent mitogen for renal epithelial cells and may contribute to renal regeneration through activation of P13/Akt pathway.
- Subjects
EPIDERMAL growth factor; REGENERATION (Biology); EPITHELIAL cells; KIDNEYS; PROTEIN kinases; MITOGENS
- Publication
FASEB Journal, 2007, Vol 21, Issue 5, pA437
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fasebj.21.5.a437-b