We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
RTN3 Regulates the Expression Level of Chemokine Receptor CXCR4 and is Required for Migration of Primordial Germ Cells.
- Authors
Haitao Li; Rong Liang; Yanan Lu; MengxiaWang; Zandong Li
- Abstract
CXCR4 is a crucial chemokine receptor that plays key roles in primordial germ cell (PGC) homing. To further characterize the CXCR4-mediated migration of PGCs, we screened CXCR4-interacting proteins using yeast two-hybrid screening. We identified reticulon3 (RTN3), a member of the reticulon family, and considered an apoptotic signal transducer, as able to interact directly with CXCR4. Furthermore, we discovered that the mRNA and protein expression levels of CXCR4 could be regulated by RTN3. We also found that RTN3 altered CXCR4 translocation and localization. Moreover, increasing the signaling of either CXCR4b or RTN3 produced similar PGC mislocalization phenotypes in zebrafish. These results suggested that RTN3 modulates PGC migration through interaction with, and regulation of, CXCR4.
- Subjects
RETICULON proteins; CHEMOKINE receptors; CELLULAR signal transduction; MESSENGER RNA; GERM cells; PROTEIN expression; CELL migration
- Publication
International Journal of Molecular Sciences, 2016, Vol 17, Issue 4, p382
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms17040382