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- Title
Two active and differently N-glycosylated isoforms of human ST3Gal-V are produced from the placental mRNA variant by a leaky scanning mechanism
- Authors
Zava, Stefania; Milani, Simona; Sottocornola, Elena; Berra, Bruno; Colombo, Irma
- Abstract
Abstract: Previously, we identified a human ST3Gal-V mRNA variant peculiarly characterized by the presence of a translational start codon localized up-stream and in-frame with the one that is usually considered as unique translation initiation site in the human gene. In this study we demonstrate, by cDNA transfection experiments, mutational analyses, enzyme activity assays, and endoglycosidase-H treatments, that the in vivo expression of this transcript gives rise to two human ST3Gal-V isoforms with distinct characteristics. Produced by a leaky scanning mechanism, they carry different N-glycan structures and exhibit differences in their GM3 synthase activity that might be relevant for the modulation of GM3 cellular content.
- Subjects
GLYCOSYLATION; MESSENGER RNA; GENETIC mutation; ANTISENSE DNA; ENZYME-linked immunosorbent assay; PLACENTA; GENETIC transcription; GENE expression
- Publication
FEBS Letters, 2010, Vol 584, Issue 8, p1476
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2010.02.062