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- Title
The vacuolar V<sub>1</sub>/V<sub>0</sub>-ATPase is involved in the release of the HOPS subunit Vps41 from vacuoles, vacuole fragmentation and fusion
- Authors
Takeda, Kozue; Cabrera, Margarita; Rohde, Jan; Bausch, Dirk; Jensen, Ole N.; Ungermann, Christian
- Abstract
Abstract: At yeast vacuoles, phosphorylation of the HOPS subunit Vps41 depends on the Yck3 kinase. In a screen for mutants that mimic the yck3Δ phenotype, in which Vps41 accumulates in vacuolar dots, we observed that mutants in the V0-part of the V0/V1-ATPase, in particular in vma16Δ, also accumulate Vps41. This accumulation is not due to a phosphorylation defect, but to reduced release of Vps41 from vma16Δ vacuoles. One reason could be a connection to vacuole fission, which is blocked in V-ATPase mutants. Vacuole fusion is not impaired between vacuoles lacking the V0-subunits Vma16 or Vma6 and wild-type vacuoles, whereas fusion between mutant vacuoles is reduced. Our data suggest a connection between vacuole biogenesis and membrane fusion.
- Subjects
YEAST; PHOSPHORYLATION; PLANT vacuoles; ADENOSINE triphosphatase
- Publication
FEBS Letters, 2008, Vol 582, Issue 10, p1558
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2008.03.055