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- Title
Regulation of histone acetyltransferase activity of p300 and PCAF by proto-oncogene protein DEK
- Authors
Ko, Soo-Il; Lee, In-Seon; Kim, Ji-Young; Kim, Sung-Mi; Kim, Dong-Wook; Lee, Kyu-Sun; Woo, Kyung-Mi; Baek, Jeong-Hwa; Choo, Jong-Kil; Seo, Sang-Beom
- Abstract
Abstract: The proto-oncogene protein DEK has been implicated in the t(6;9) chromosomal translocation associated with a subtype of acute myelogenous leukemia (AML), which results in the formation of a DEK-CAN fusion protein. Histone acetylation is an important post-translational modification which is involved in transcriptional regulation. In this study, we report that the acidic domain containing protein DEK interacts with histones and exerts a potent inhibitory effect on both p300 and PCAF-mediated histone acetyltransferase activity and transcription. Using chromatin immunoprecipitation assays, we have demonstrated that the recruitment of DEK to the appropriate promoter induces the histone H3 and H4 hypoacetylation of chromatin. Collectively, our data illustrate the important regulatory role played by protein DEK in transcriptional regulation, and suggest that transcription-regulating acidic domain regions may play a role in leukemogenesis.
- Subjects
CHROMATIN; DNA polymerases; ACUTE myeloid leukemia; GENETIC translation
- Publication
FEBS Letters, 2006, Vol 580, Issue 13, p3217
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2006.04.081