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- Title
ADP-ribosylation factor (ARF)-like 7 (ARL7) is induced by cholesterol loading and participates in apolipoprotein AI-dependent cholesterol export
- Authors
Engel, Thomas; Lueken, Aloys; Bode, Günther; Hobohm, Uwe; Lorkowski, Stefan; Schlueter, Bernhard; Rust, Stephan; Cullen, Paul; Pech, Michael; Assmann, Gerd; Seedorf, Udo
- Abstract
Here, we identify ADP-ribosylation factor (ARF)-like 7 (ARL7) as the only ARF- and ARL-family member whose mRNA-expression is induced by liver X-receptor/retinoid X-receptor agonists or cholesterol loading in human macrophages. Moreover, subcellular distribution of mutant and wild type ARL7-enhanced green fluorescent protein (EGFP) supports that ARL7 may be involved in a vesicular transport step between a perinuclear compartment and the plasma membrane. Therefore, we investigated the effect of ARL7 over-expression on the cholesterol secretory pathway. We found that expression of wild type and dominant active ARL7-EGFP stimulated the rate of apolipoprotein AI-specific cholesterol efflux 1.7- and 2.8-fold. In contrast, expression of the dominant negative form of ARL7-EGFP led to ∼50% inhibition of cholesterol efflux. This data is consistent with a model in which ARL7 is involved in transport between a perinuclear compartment and the plasma membrane apparently linked to the ABCA1-mediated cholesterol secretion pathway.
- Subjects
CHOLESTEROL; PROTEINS; APOLIPOPROTEINS; POLYMERASE chain reaction
- Publication
FEBS Letters, 2004, Vol 566, Issue 1-3, p241
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2004.04.048