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- Title
Activation and Stabilization of Olive Recombinant 13-Hydroperoxide Lyase Using Selected Additives.
- Authors
Jacopini, Sabrina; Vincenti, Sophie; Mariani, Magali; Brunini-Bronzini de Caraffa, Virginie; Gambotti, Claude; Desjobert, Jean-Marie; Muselli, Alain; Costa, Jean; Tomi, Félix; Berti, Liliane; Maury, Jacques
- Abstract
The stabilization of olive recombinant hydroperoxide lyases (rHPLs) was investigated using selected chemical additives. Two rHPLs were studied: HPL full-length and HPL with its chloroplast transit peptide deleted (matured HPL). Both olive rHPLs are relatively stable at 4 °C, and enzyme activity can be preserved (about 100% of the rHPL activities are maintained) during 5 weeks of storage at −20 or at −80 °C in the presence of glycerol (10%, v/ v). Among the additives used in this study, glycine (2.5% w/ v), NaCl (0.5 M), and NaSO (0.25 M) provided the highest activation of HPL full-length activity, while the best matured HPL activity was obtained with NaSO (0.25 M) and NaCl (1 M). Although the inactivation rate constants ( k) showed that these additives inactivate both rHPLs, their use is still relevant as they strongly increase HPL activity. Results of C6-aldehyde production assays also showed that glycine, NaCl, and NaSO are appropriate additives and that NaCl appears to be the best additive, at least for hexanal production.
- Subjects
HYDROPEROXIDE lyase; ADDITIVES; CHLOROPLASTS; GLYCERIN; ALDEHYDES
- Publication
Applied Biochemistry & Biotechnology, 2017, Vol 182, Issue 3, p1000
- ISSN
0273-2289
- Publication type
Article
- DOI
10.1007/s12010-016-2377-0