We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Characterization and genomic analysis of a novel bacteriophage BUCT_49532 lysing Klebsiella pneumoniae.
- Authors
Zhang, Guangye; Liu, Yucong; Wang, Jinhong; Li, Nan; Han, Pengjun; Chen, Yiming; Xu, Weijian; Liu, Changxia
- Abstract
Bacteriophages are a type of virus widely distributed in nature that demonstrates a remarkable aptitude for selectively recognizing and infecting bacteria. In particular, Klebsiella pneumoniae is acknowledged as a clinical pathogen responsible for nosocomial infections and frequently develops multidrug resistance. Considering the increasing prevalence of antibiotic-resistant bacteria, bacteriophages have emerged as a compelling alternative therapeutic approach. In this study, a novel phage named BUCT_49532 was isolated from sewage using K. pneumoniae K1119 as the host. Electron microscopy revealed that BUCT_49532 belongs to the Caudoviricetes class. Further analysis through whole genome sequencing demonstrated that BUCT_49532 is a Jedunavirus comprised of linear double-stranded DNA with a length of 49,532 bp. Comparative genomics analysis based on average nucleotide identity (ANI) values revealed that BUCT_49532 should be identified as a novel species. Characterized by a good safety profile, high environmental stability, and strong lytic performance, phage BUCT_49532 presents an interesting case for consideration. Although its host range is relatively narrow, its application potential can be expanded by utilizing phage cocktails, making it a promising candidate for biocontrol approaches.
- Subjects
KLEBSIELLA pneumoniae; BACTERIOPHAGES; GENOMICS; WHOLE genome sequencing; COMPARATIVE genomics; BACTERIOPHAGE typing; BIOLOGICAL pest control agents; BIOLOGICAL weed control
- Publication
Virus Genes, 2023, Vol 59, Issue 6, p852
- ISSN
0920-8569
- Publication type
Article
- DOI
10.1007/s11262-023-02033-8