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- Title
Screening of Wound-Responsive Genes Identifies an Immediate-Early Expressed Gene Encoding a Highly Charged Protein in Mechanically Wounded Tobacco Plants.
- Authors
Hara, Kojiro; Yagi, Mitsue; Koizumi, Nozomu; Kusano, Tomonobu; Sano, Hiroshi
- Abstract
In order to identify genes that are temporally and spatially regulated during wound response, a cDNA population in mechanically wounded tobacco leaves was screened by the fluorescence differential display method. Of 28 clones initially identified to have altered levels of transcripts within 3 h of wounding, eight were characterized. Although each clone showed a unique pattern of transcript accumulation, one distinct clone was further characterized because of its immediate-early response. Its transcripts began to accumulate 10 min after wounding, reached a maximum level within 1 h and disappeared after 2 h. The response, which occurred repeatably and systemically, was observed by the treatment with propionic acid or erythrosin B, indicating that cytosolic acidification could be one of the signals for immediate-early response of this gene. The cDNA encodes a polypeptide of 513 amino acids with a relative molecular mass of 60,952. The putative polypeptide is rich in lysine (K), glutamic acid (E) and aspartic acid (D), which constitute up to 70% of total amino acids, and was therefore designated as KED. The KED polypeptide is composed of a highly hydrophilic Nterminal region and a relatively hydrophobic C-terminal region, suggesting that KED may function through electrostatic interactions with cellular components.
- Subjects
GENETIC code; GENE expression in plants; TOBACCO; PLANT injuries; PLANT mechanics; ANTISENSE DNA; POLYPEPTIDES; PLANT proteins
- Publication
Plant & Cell Physiology, 2000, Vol 41, Issue 6, p684
- ISSN
0032-0781
- Publication type
Article
- DOI
10.1093/pcp/41.6.684