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- Title
A Non-invasive Imageable GFP-expressing Mouse Model of Orthotopic Human Bladder Cancer.
- Authors
YU SUN; NISHINO, HIROTO; MING ZHAO; MIYAKE, KENTARO; SUGISAWA, NORIHIKO; JUN YAMAMOTO; TASHIRO, YOSHIHIKO; INUBUSHI, SACHIKO; KAZUYUKI HAMADA; GUANGWEI ZHU; HYEIN LIM; HOFFMAN, ROBERT M.
- Abstract
Background/Aim: A more realistic mouse model of bladder cancer is necessary to develop effective drugs for the disease. Tumor models enhanced by bright fluorescent-reporter genes to follow the disease in real-time would enhance the ability to accurately predict the efficacy of various therapeutics on this particularly-malignant human cancer. Materials and Methods: A highly-fluorescent green fluorescent protein (GFP)- expressing bladder cancer model was orthotopically established in nude mice using the UM-UC-3 human bladder-cancer cell line (UM-UC-3-GFP). Fragments from a subcutaneous tumor of UM-UC-3-GFP were surgically implanted into the nude mouse bladder. Non-invasive and intra-vital fluorescence imaging was obtained with a simple imaging box. Results: The GFP-expressing orthotopic bladder tumor was imaged in realtime non-invasively as well as intra-vitally, with the two methods correlating at r=0.99. Conclusion: This is the first noninvasive-fluorescence-imaging orthotopic model of bladder cancer and can be used for rapidly screening novel effective agents for this recalcitrant disease.
- Subjects
BLADDER cancer treatment; GREEN fluorescent protein; TUMOR transplantation; CANCER cells; CANCER treatment; ANIMAL models of cancer
- Publication
In Vivo, 2020, Vol 34, Issue 6, p3225
- ISSN
0258-851X
- Publication type
Article
- DOI
10.21873/invivo.12158