We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Box-Behnken Design for Optimizing Ultrasonic-Assisted Enzymatic Extraction of Soluble Dietary Fiber from Pleurotus citrinopilestus.
- Authors
Yu, Panling; Yu, Changxia; Chen, Mingjie; Dong, Qin; Hu, Die; Zhang, Baosheng; Zhang, Mengke; Ma, Jianshuai; Xu, Baoting; Zhao, Yan
- Abstract
Pleurotus citrinopilestus contains a variety of physiologically and pharmacologically active compounds. A key active component among these compounds is dietary fiber, a polysaccharide that exhibits several biological properties. The objective of this study was to assess how soluble dietary fiber (SDF) from Pleurotus citrinopilestus responded to ultrasonic-assisted enzymatic (UAE) extraction. The response surface method (RSM) combined with the Box-Behnken design method (BBD) was used to optimize the yield of SDF. The effects of the liquid-solid ratio (35–55 mL/g), α-amylase concentration (0.5–2.5%), complex protease concentration (0.4–2.0%), and ultrasonication time (15–55 min) on the yield of SDF were examined. The RSM results revealed the optimal liquid-solid ratio (45 mL/g), α-amylase concentration (1.5%), complex protease concentration (1.2%), and ultrasonic time (35 min). The SDF yield was 10.25%, which is close to the predicted value (10.08%).
- Subjects
DIETARY fiber; PLEUROTUS; BIOACTIVE compounds; AMYLASES; POLYSACCHARIDES; PROTEOLYTIC enzymes
- Publication
Horticulturae, 2023, Vol 9, Issue 12, p1322
- ISSN
2311-7524
- Publication type
Article
- DOI
10.3390/horticulturae9121322