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- Title
Chlorpyrifos and endosulfan affect buffalo oocyte maturation, fertilization, and embryo development in vitro directly and through cumulus cells.
- Authors
Nandi, S.; Gupta, P. S. P.; Roy, S. C.; Selvaraju, S.; Ravindra, J. P.
- Abstract
This study was undertaken to examine the effect of 10 different levels (0, 0.005, 0.01, 0.02, 0.05, 0.1, 0.5, 1.0, 2.0, and 4.0 μg/mL) of two pesticides (chlorpyrifos and endosulfan) on buffalo oocyte viability, maturation, fertilization, and developmental competences in vitro. Studies were conducted to test the development of oocytes cultured with pesticides during maturation, fertilization, and during different embryo development stages. We also conducted experiments to test the hypotheses that the effects of these pesticides are hormones and somatic cells mediated. We observed a dose dependant decline in viability and developmental competence rates of oocytes. Chlorpyrifos and endosulfan had a negative impact on oocytes at 0.02 and 0.1 μg/mL levels, respectively. These pesticides reduced the oocyte nuclear maturation by a direct effect on oocytes, cumulus cell-mediated action, and by blocking the action of hormones. Chlorpyrifos was found to be more ovotoxic and embryotoxic than endosulfan. This study will provide information on dose-response relationship and risk assessment in domestic buffaloes. © 2009 Wiley Periodicals, Inc. Environ Toxicol 26: 57-67, 2011.
- Subjects
CHLORPYRIFOS; ENDOSULFAN; WATER buffalo; OOGENESIS; FERTILIZATION in vitro; LIVESTOCK embryos; PESTICIDES &; wildlife; PESTICIDE toxicology; REPRODUCTIVE toxicology; REPRODUCTION
- Publication
Environmental Toxicology, 2011, Vol 26, Issue 1, p57
- ISSN
1520-4081
- Publication type
Article
- DOI
10.1002/tox.20529