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- Title
腈水合酶的稳定性改造研究进展.
- Authors
沈瑞华; 郭军玲; 周哲
- Abstract
JNiLrile hvdraLasc (JNllasc, EC 4. 2. 1. 84) is a key mcLallocnzymc in Lhc hvdraLion of niLriles Lo Lheir corresponding amides LhaL arc widely used in Lhe indusLrial pindncLion of highly value amides. However,Lhc poor LhermosLaliliLy of JNllase is Lhc major facLor prevenLing iLs exLensive indusLrial applicaLion. Wc summarize here Lhe molecLilar modificaLion meLhods Lo improve Lhc sLabiliLv of Ml丄asc, including salL bridge inserLion, free energy rcdnclion, homologous fragmenL rccombinaLion, and gene fusion. These achievemenLs noL only improve Lhe dcvelopmcnL of biocaLalvLic prod Lie Lion of amide compounds,buL also provide LheoreLical siipporL for JNllase pro Lein engineering.
- Publication
Chinese Journal of Bioprocess Engineering, 2020, Vol 18, Issue 3, p269
- ISSN
1672-3678
- Publication type
Article
- DOI
10.3969/j.issn.1672-3678.2020.03.001