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- Title
Development of Lateral Flow Immunoassay for Antigen Detection in Human Angiostrongylus cantonensis Infection.
- Authors
Mu-Xin Chen; Jia-Xu Chen; Shao-Hong Chen; Da-Na Huang; Lin Ai; Ren-Li Zhang
- Abstract
Angiostrongyliasis is difficult to be diagnosed for the reason that no ideal method can be used. Serologic tests require specific equipment and are not always available in poverty-stricken zone and are time-consuming. A lateral flow immunoassay (LFIA) may be useful for angiostrongyliasis control. We established a LFIA for the diagnosis of angiostrongyliasis based on 2 monoclonal antibodies (mAbs) against antigens of Angiostrongylus cantonensis adults. The sensitivity and specificity were 91.1% and 100% in LFIA, while those of commercial ELISA kit was 97.8% and 86.3%, respectively. Youden index was 0.91 in LFIA and 0.84 in commercial ELISA kit. LFIA showed detection limit of 1 ng/ml of A. cantonensis ES antigens. This LFIA was simple, rapid, highly sensitive and specific, which opened an alternative approach for the diagnosis of human angiostrongyliasis.
- Subjects
IMMUNOASSAY; HELMINTH antigens; ANGIOSTRONGYLUS cantonensis; MONOCLONAL antibodies; ENZYME-linked immunosorbent assay; SENSITIVITY analysis
- Publication
Korean Journal of Parasitology, 2016, Vol 54, Issue 3, p375
- ISSN
0023-4001
- Publication type
Article
- DOI
10.3347/kjp.2016.54.3.375