We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Fine-mapping of a locus on linkage group 23 for sex determination in Nile tilapia ( Oreochromis niloticus).
- Authors
Eshel, O.; Shirak, A.; Weller, J. I.; Slossman, T.; Hulata, G.; Cnaani, A.; Ron, M.
- Abstract
Genetic markers in tilapia species associated with loci affecting sex determination (SD), sex-specific mortality or both were mapped to linkage groups (LG) 1, 2, 3, 6 and 23. The objective of this study was to use these markers to fine-map the locus with the greatest effect on SD in Oreochromis niloticus. Our parental stock, full-sibs of Nile tilapia (Swansea origin), were divided into three groups: (i) untreated, (ii) feminized by diethylstilbestrol and (iii) masculinized by 17α-methyltestosterone. We analysed the first group for association of microsatellite markers representing these five LGs. The strongest association with gender was found on LG23 for marker UNH898 (χ; P = 8.6 × 10). Allele 276 was found almost exclusively in males, and we hypothesized that this allele is a male-associated allele (MAA). Sex-reversed individuals were used for mating experiments with and without the segregating MAA. Mating of individuals lacking the MAA resulted in all-female progeny. Mating of two heterozygotes for MAA gave rise to 81 males and 30 females. Analysis of association between gender and genotypes identified the MAA in 98.6% of males as opposed to 8.0% of females (χ; P = 2.5 × 10). Eight markers that flank UNH898 were genotyped to map the locus on LG23 within a confidence interval of 16-21 cM. Mating of homozygous individuals for MAA is underway for production of all-male populations.
- Subjects
NILE River; SEXING of fish; TILAPIA; GENETIC markers; LOCUS (Genetics)
- Publication
Animal Genetics, 2011, Vol 42, Issue 2, p222
- ISSN
0268-9146
- Publication type
Article
- DOI
10.1111/j.1365-2052.2010.02128.x