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- Title
Determining Whether Agonist Density or Agonist Number Is More Important for Immune Activation via Micoparticle Based Assay.
- Authors
Deak, Peter; Kimani, Flora; Cassaidy, Brittney; Esser-Kahn, Aaron
- Abstract
It is unknown if surface bound toll-like-receptor (TLR) agonists activate cells via density or total molecular number. To answer this question, we developed a TLR agonist surface conjugated polystyrene microparticle (MP) system. Using a library of MPs with varying TLR agonist density and number, we simultaneously observed innate immune cell MP uptake and TNFα expression using ImageStream flow cytometry on a cell by cell basis. The data shows that total TLR number and not density drives cellular activation with a threshold of approximately 105–106 TLR agonists. We believe that this information will be crucial for the design of particulate vaccine formulations.
- Subjects
ACTIVATION energy; DENSITY; FLOW cytometry; POLYSTYRENE
- Publication
Frontiers in Immunology, 2020, p1
- ISSN
1664-3224
- Publication type
Article
- DOI
10.3389/fimmu.2020.00642