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- Title
Identification of a novel glycerophosphodiester phosphodiesterase from Bacillus altitudinis W3 and its application in degradation of diphenyl phosphate.
- Authors
Ren, Runxian; Zhai, Lixin; Tian, Qiaopeng; Meng, Di; Guan, Zhengbin; Cai, Yujie; Liao, Xiangru
- Abstract
Diphenyl phosphate (DPHP) has been increasingly detected in environmental samples, posing a potential hazard to humans and other organisms and arousing concern regarding its adverse effects. Biological degradation of DPHP is considered a promising and environmentally friendly method for its removal. In this study, the bagdpd gene was mined from the Bacillus altitudinis W3 genome and identified as a glycerophosphodiester phosphodiesterase by bioinformatics analysis. The enzyme was expressed and its biochemical properties were studied. When using bis(4-nitrophenyl) phosphate as substrate, enzyme activity was optimal at 55 °C and a pH of 8.5. The enzyme remained stable in the pH range of 8.0 − 10.0. The rBaGDPD enzyme degraded DPHP and the reaction product was identified as phenyl phosphate by LC–MS. This is the first report of a glycerophosphodiester phosphodiesterase exhibiting hydrolytic activity against DPHP. This study demonstrated that rBaGDPD could have the potential for bioremediation and industrial applications.
- Subjects
DIPHENYL; BIODEGRADATION; PHOSPHATES; ENVIRONMENTAL sampling; GENES
- Publication
3 Biotech, 2021, Vol 11, Issue 4, p1
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-021-02704-z