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- Title
Monoclonal antibodies to early pregnancy factor perturb tumour cell growth.
- Authors
Quinn, K. A.; Athanasas-Platsis, S.; Wong, T.-Y.; Rolfe, B. E.; Cavanagh, A. C.; Morton, H.
- Abstract
The pregnancy-associated substance early pregnancy factor (EPF) has previously been reported as a product of tumours of germ cell origin. More recently EPF (or an EPF-related substance, tEPF) has also been detected in the serum of patients bearing tumours of non-germ cell origin. We report here the production of tEPF by a variety of cultured transformed and tumour cell lines, of both germ and non-germ cell origin. Antibodies specific for EPF remove all tEPF activity from tumour cell conditioned medium. tEPF production is found to be associated with cell division; tEPF is no longer detected after growth arrest or differentiation. Co-culture of tumour cells with increasing doses of anti-EPF monoclonal antibodies resulted in a significant, dose-dependent decrease in rate of cell growth and viability. Similar anti-EPF concentrations had no effect on the concanavalin A induced proliferation of mouse spleen cells. These studies suggest, therefore, that tEPF is a growth-regulated product of cultured tumour and transformed cells. These cells are also dependent upon tEPF for continued growth, i.e. tEPF is acting in the autocrine mode.
- Subjects
TUMORS; SERUM; GERM cells; CELL lines; IMMUNOGLOBULINS; MONOCLONAL antibodies; GROWTH factors
- Publication
Clinical & Experimental Immunology, 1990, Vol 80, Issue 1, p100
- ISSN
0009-9104
- Publication type
Article
- DOI
10.1111/1365-2249.ep15950759