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- Title
Extensive intraspecific polymorphism detected by SSCP at the nuclear C- mos gene in the endemic Iberian lizard Lacerta schreiberi.
- Authors
GODINHO, RAQUEL; DOMINGUES, VERA; CRESPO, EDUARDO G.; FERRAND, NUNO
- Abstract
C- mos is a highly conserved intronless gene that has proved useful in the analysis of ancient phylogenetic relationships within vertebrates. We selected the Iberian endemic Schreiber's green lizard ( Lacerta schreiberi) that persisted in allopatric refugia since the late Pliocene to investigate the utility of the C- mos nuclear gene for intraspecific phylogeographic studies. Our combination of DNA sequencing with the high resolving power of single-strand conformational polymorphism (SSCP) effectively discriminated four common alleles showing strong population structuring ( FST = 0.46). In addition, reconstruction of allele phylogenetic relationships further improved our understanding of C- mos spatial patterns of variation and allowed a comparison with previously described mitochondrial DNA data. Finally, limited sequencing of an extended C- mos fragment in six additional Lacerta species showed extensive polymorphism, to our knowledge representing a rare example of variation in a highly conserved nuclear gene.
- Subjects
LACERTA; GENETIC polymorphisms; LIZARDS; LACERTIDAE; ANIMAL genetics; PHYLOGENY; VERTEBRATES; PHYLOGEOGRAPHY; ANIMAL population genetics; SPECIES
- Publication
Molecular Ecology, 2006, Vol 15, Issue 3, p731
- ISSN
0962-1083
- Publication type
Article
- DOI
10.1111/j.1365-294X.2006.02813.x